Blood is drawn from patient, to collect one RegenBCT tube (or other PRP specific tube) ~10mL of blood, tube must be inverted/mixed 25 times and then is centrifuged to separate plasma components. Plasma is extracted (~4-6 mL) and drawn up into syringes.
BLT/topical numbing cream can be applied 10-20 min prior to procedure to aid in reducing discomfort; cream should be wiped off, and the skin is cleansed with soap and water, then pat dry. Area is then cleansed thoroughly with rubbing alcohol and practitioner should be very conscientious of maintaining a sterile field during the treatment.
When procedure is ready to commence, the skin is then prepped with some plasma topically and a microneedling approved lubricating gel (ex: Skinceuticals B5 Hydrating gel) is applied to the skin before the microneedling device can be used. If patient is having their first treatment, recommended settings are: 0.5mm depth to forehead, 0.25mm depth to periocular area and nose, 0.5-1mm depth on rest of face, and 0.5mm depth on neck and chest. Practitioners may use various micro needling techniques such as stamping, or various sweeps in diversified patterns, depending on the practitioner’s preference or the micro needling device. You want to achieve uniform mild erythema and pinpoint bleeding as your endpoint. Though your subsequent sessions, depth of treatment can be increased as needed, as most microneedling devices can achieve up to 2.5mm depths, which are useful when treating atrophic scars, but always should be gradually increased to measure patients tolerability and response.
Upon completion of the micro needling treatment, another layer of plasma is applied topically (0.5-1mL total), and a micro needling approved vitamin C serum/antioxidants (ex: Skinceuticals CE Ferrulic) may also be applied topically. (It is important to only apply topical products that have been studied in conjunction with microneedling to avoid having a granulomatous reaction from occurring.)
Lastly, if looking for a better rhytid correction or volume replacement, mid dermal injection of the plasma serum can be used, injections are done similar to the various HA filler techniques, injected directly into targeted areas such as periorbital & perioral rhytids, nasal labial folds, marionette lines, accordion lines, forehead rhytids, horizontal neck lines, and also can be fanned across the décolletage to soften the severity of wrinkles, improve texture, and may aid to improve hyperpigmented skin. It is very important to use safe injection techniques, such as slow, retrograde injection techniques and a 26 gauge needle to avoid possible intravascular occlusion, and to apply firm pressure to tissue post injection to minimize purpura.
At least 3 treatments are recommended, one month apart to obtain, to obtain desired results, and one treatment, every 6 months for maintenance.